Tau Uptake and Seeding Assay from Creative Biolabs's blog

Background of Tau Uptake and Seeding

Tau proteins are a group of microtubules associated proteins that are mainly located in the central nervous system astrocytes. Tau proteins participate in several biological activities, including regulation of microtubule stabilization and assembly, maintenance of neuronal cytoskeleton, iron export, and axonal transportation. However, tau proteins turn into hyperphosphorylated insoluble aggregates could lead to neurodegenerative tauopathies, such as Alzheimer's disease (AD). The aggregation of tau may spread throughout the cortex by pathological tau uptake and seeding within neurons. The development of therapeutics for AD that can suppress or prevent the uptake and seeding of tau is coming into perspective. As a result, reliable in vitro assay services offered by Creative Biolabs is able to recur tau uptake and seeding to identify appropriate target epitopes.

Tau Uptake and Seeding Assay at Creative Biolabs

Distinct target epitopes against tau include N-terminal antibodies, phospho-tau–specific antibodies, and conformation-dependent antibodies. Exploring suitable target epitopes and antibodies against tau involved in uptake and seeding, in vitro assays are required that are capable of determining the effect of antibodies on tau uptake and seeding in neurons. Creative Biolabs offers tau uptake assay services to quantify the neuronal tau uptake. You can get an overview of our workflow in the following scheme.