Adnectin-based CAR-T cell from Creative Biolabs's blog

Adnectin can be used as the antigen recognition domain in CAR constructs instead of scFv. We have designed multiple antigen-specific adnectin-CARs. Firstly, mRNA display is used to generate different adnectin clones, which target the target extracellular domain with different affinities. Then, different clones of target-specific adnectins were cloned upstream of the hinge domain to replace the scFv sequence. The adnectin-based extracellular domain is connected by the CD8α hinge and transmembrane domain, CD28 and 4-1BB costimulatory domains, and CD3ζ T cell receptor signaling domain. It is worth noting that we also provide adnectin-based first-generation and second-generation CARs.